ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (2): 212-217.doi: 10.11843/j.issn.0366-6964.2014.02.007

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Research on L-type Amino Acid Transporter 1 and Its Ligand Coordinated Expression in Dairy Cow Mammary Gland

LIN Ye,HOU Xiao-ming*   

  1. (The Key Laboratory of Dairy Science of Ministry of Education,Northeast Agricultural University,Harbin 150030, China)
  • Received:2013-08-20 Online:2014-02-23 Published:2014-02-23

Abstract:

To investigate the underlying relationship between amino acid transportation and lactation in dairy cow mammary gland,LAT1 and 4F2hc expression were detected by laser confocal microscope.The results showed that LAT1 and 4F2hc expression were higher in mammary gland with high quality milk than mammary gland with low quality milk and mammary gland in dry period.In lactation,LAT1 and 4F2hc were detected on the basolateral of acinar epithelial cells and myoepithelial cells.In dry period,LAT1 and 4F2hc were detected on the plasma membrane of ductal epithelial cells.Correlation analysis showed LAT1 expression was highly correlated with 4F2hc.All these results revealed that LAT1 was the major amino acid transporter in mammary gland of dairy cow.LAT1/4F2hc coordinated expression was closely related to milk protein synthesis and mammary gland reconstruction.

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